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doi: 10.1371/journal.ppat.1011371 82 FongGHTakedaK

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The 28-day use window applies only to refrigerated storage

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Interestingly, the expression of GHR and IGF-1 were reduced in the skeletal muscle of these animals

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The separated proteins were then transferred onto a PVDF membrane (Merck Millipore) and blocked using skim milk for 2 h at room temperature

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This study received ethical committee approval from the Ethics Committee of the Second Hospital of Shandong University with written informed consent obtained from each participant prior to enrollment

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Moreover, elevated AR activity may impair antioxidant defences through NADPH depletion, thereby limiting glutathione (GSH) regeneration and exacerbating oxidative stress 10,52 ( Figure 7 )

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